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Centre for Energy and Environmental Technologies ›Institute of Environmental Technology ›Science and research ›Research groups ›Biochemical Processes

Biochemical Processes

This laboratory focuses on both basic and applied research, using biomass (including biowaste) to produce biowaste, and the research of the subsequent processes, such as biogas purification. We do modelling and evaluation of the processes of anaerobic transformation of biomass into biogas and digestate, which may both be used in energy production. We provide basic analytical service for biogas stations and we also evaluate biogas technologies. We test methane production from non-traditional substrate. We also investigate the biomass aerobic degradation process, i.e. composting. New bioreactors, especially lower capacity ones, are being developed.

Research activities

  • Optimization of wet and dry anaerobic digestion.
  • Assessment of anaerobic digestion of demanding materials and waste streams.
  • Design and development of novel bioreactors – focus on mobile and easily transferable units.

Equipment

  • Respirometric system WTW OXITOP AN6.
  • Series of 1lt bottle reactors (BMP tests)
  • Lab-scale rotary reactor (15 lt)
  • Four (4) vertical reactors coupled with incubator (20 lt)
  • Three (3) CSTR reactors (60 lt)
  • Two (2) rotary reactors with volume 160 lt
  • Two-stage vertical AD reactor (200 lt)
  • Pilot-scale horizontal CSTR AD unit (400 lt)
  • Adjustable inclination (0-90°) pilot-scale reactor (900 lt)
  • Pilot-scale rotary reactor with 10 m3 volume
  • Infrared and electrochemical biogas analysers (CH4, CO2, O2, H2, H2S).
  • Elemental analyser (C, H, N, O, S).
  • Measuring equipment: COD, pH, conductivity, moisture, ORP, O2.
  • Processing equipment: incubators, dryers, autoclaves, gas-holders, crushers, mills, homogenizers.

Offered services

  • Physicochemical characterization of solids (biomass, biowaste, digestate).
  • Chemical characterization of gases (biogas, natural gas).
  • Feedstock preparation and pre-treatment (crushing, hydrolysis, acidification).
  • Downscale of industrial plants anaerobic (co)digestion process.
  • Testing of industrial process parameters (loading, retention time, mixing).
  • Assessment of additives (trace elements, buffering, enzymes, microorganisms).

Research team

Ing. Jiří Rusín, Ph.D.
Ing. et Ing. Kateřina Chamrádová, Ph.D.
Ing. Markéta Bouchalová, Ph.D.
Ing. Basinas Panagiotis, Ph.D.
Ing. Petra Wojnarová
Ing. Tomáš Výtisk, Ph.D.
Jiří Jureček

Contact

Ing. Jiří Rusín, Ph.D.
e-mail:
tel.: 00420 597 32 7328

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Publishing and scientific activity

Article

VRÁBLOVÁ, Martina, Kateřina SMUTNÁ, Kateřina CHAMRÁDOVÁ, Daniel VRÁBL, Ivan KOUTNÍK, Jiří RUSÍN, Markéta BOUCHALOVÁ, Anna GAVLOVÁ, Hana SEZIMOVÁ, Martin NAVRÁTIL, Richard CHALUPA, Barbora TENKLOVÁ and Jitka PAVLÍKOVÁ. Co-composting of sewage sludge as an effective technology for the production of substrates with reduced content of pharmaceutical residues. Science of the Total Environment. New York: Elsevier Science, 2024, 915(MAR 10 2024), p. nestránkováno. ISSN 0048-9697. [Detail]
BASINAS, Panagiotis, Kateřina CHAMRÁDOVÁ, Olga VOSNAKI and Jiří RUSÍN. ENHANCEMENT OF BIOGAS GENERATION BY UTILIZING RAW AND MODIFIED WITH HNO3 BIOCHAR OBTAINED FROM PYROLYSIS OF BIOMASS AND DIGESTATE. Detritus. Cisa Publisher, 2023, 2023(24), p. 28-39. ISSN 2611-4127. [Detail]
BASINAS, Panagiotis, Jiří RUSÍN, Kateřina CHAMRÁDOVÁ and S.P. KALDIS. Pyrolysis of the anaerobic digestion solid by-product: Characterization of digestate decomposition and screening of the biochar use as soil amendment and as additive in anaerobic digestion. Energy Conversion and Management. Oxford: PERGAMON-ELSEVIER SCIENCE LTD, 2023, 277(1 February 2023), p. 1-12. ISSN 0196-8904. [Detail]
WOJNAROVÁ, Petra, Jiří RUSÍN, Panagiotis BASINAS, Martin KOŠTEJN, Josef NĚMEC, Petr STANOVSKÝ, Albert S. KIM and Pavel IZÁK. Unveiling the potential of composite water-swollen spiral wound membrane for design of low-cost raw biogas purification. Separation and Purification Technology. Elsevier, 2023, 326(-), p. 1-9. ISSN 1383-5866. [Detail]
BOUCHALOVÁ, Markéta, Jiří RUSÍN, Panagiotis BASINAS, Kamil Maciej GÓRECKI, Roman BURYJAN and Hana FRANKOVÁ. Biogas generation potential of starch-and polylactide- based biodegradable plastics. Waste Forum. České ekologické manažerské centrum, 2023, p. 21-31. ISSN 1804-0195. [Detail]
RUSÍN, Jiří, Kateřina CHAMRÁDOVÁ, Tomáš JASTRZEMBSKI and Jan SKŘÍNSKÝ. Explosion Characteristics of a Biogas/air Mixtures. Chemical Engineering Transactions. Milano: The Italian Association of Chemical Engineering, 2022, 90(02/2022), p. 271-276. ISBN 978-88-95608-27-3. [Detail]
WOJNAROVÁ, Petra, Panagiotis BASINAS, Jiří RUSÍN and Roman BURYJAN. Biogas upgrading using a water-swollen composite polyamide membrane. Waste Forum. České ekologické manažerské centrum, 2022, 4(4), p. 263-270. ISSN 1804-0195. [Detail]
BASINAS, Panagiotis, Jiří RUSÍN, Kateřina CHAMRÁDOVÁ, Kateřina MALACHOVÁ, Zuzana RYBKOVÁ and Čeněk NOVOTNÝ. Fungal pretreatment parameters for improving methane generation from anaerobic digestion of corn silage. Bioresource technology. San Diego: Elsevier, 2021, 345(February 2022), p. nestránkováno. ISSN 0960-8524. [Detail]
KODYMOVÁ, Jana, Silvie HEVIÁNKOVÁ, Miroslav KYNCL and Jiří RUSÍN. Testing the impact of the waste product from the biogas plants on plant germination and initial root growth. Inżynieria Mineralna. Polskie towarzystwo przeróbki kopalin, 2021, 1(47), p. 25-31. ISSN 1640-4920. [Detail]
PLATOŠOVÁ, Daniela, Jiří RUSÍN, Jan PLATOŠ, Kateřina SMUTNÁ and Roman BURYJAN. Case study of anaerobic digestion process stability detected by dissolved hydrogen concentration. Processes. Basilej: MDPI Open Access Publishing, 2021, 9(1), p. 1-11. ISSN 2227-9717. [Detail]
BASINAS, Panagiotis, Jiří RUSÍN and Kateřina CHAMRÁDOVÁ. Dry anaerobic digestion of the fine particle fraction of mechanically-sorted organic fraction of municipal solid waste in laboratory and pilot reactor. Waste Management. Oxford: PERGAMON-ELSEVIER SCIENCE LTD, 2021, 136(12/2021), p. 83-92. ISSN 0956-053X. [Detail]
BASINAS, Panagiotis, Jiří RUSÍN and Kateřina CHAMRÁDOVÁ. Assessment of high-solid mesophilic and thermophilic anaerobic digestion of mechanically-separated municipal solid waste. Environmental Research. San Diego: Academic Press, 2020, 192(September), p. 1-14. ISSN 0013-9351. [Detail]
RUSÍN, Jiří, Silvia MUDRONČEKOVÁ, Marek BARTA and Kateřina CHAMRÁDOVÁ. Spruce bark beetles as a biological waste that can be processed on a biogas plant. Turkish Journal of Agriculture and Forestry. Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, 2020, 44(2), p. 169-179. ISSN 1300-011X. [Detail]
WOJNAROVÁ, Petra and Jiří RUSÍN. Tenebrio molitor as substrate for anaerobic digestion. Waste Forum. České ekologické manažerské centrum, 2020, 3(1), p. 121-130. ISSN 1804-0195. [Detail]
RUSÍN, Jiří, Panagiotis BASINAS, Markéta BOUCHALOVÁ, Kateřina CHAMRÁDOVÁ and Roman BURYJAN. An example of increasing biogas and methane production by supplying anaerobic digester with biochar prepared from solid digestate. Waste Forum. České ekologické manažerské centrum, 2020, Neuveden.(4), p. 228-237. ISSN 1804-0195. [Detail]
RUSÍN, Jiří, Kateřina CHAMRÁDOVÁ and Panagiotis BASINAS. Two-stage psychrophilic anaerobic digestion of food waste: Comparison to conventional single-stage mesophilic process. Waste Management. PERGAMON-ELSEVIER SCIENCE LTD, 2020, 119(September), p. 172-182. ISSN 0956-053X. [Detail]
RUSÍN, Jiří, Kateřina KAŠÁKOVÁ and Kateřina CHAMRÁDOVÁ. High-solids semi-continuous anaerobic digestion of corn silage in bag-type digester. Green processing and synthesis. WALTER DE GRUYTER & CO, 2018, 7(3), p. 268-276. ISSN 2191-9542. [Detail]
RUSÍN, Jiří, Kateřina CHAMRÁDOVÁ and Barbora GRYCOVÁ. The influence of biomass agitation on biogas and methane production using the high-solids thermophilic anaerobic digestion. Green processing and synthesis. Berlín: WALTER DE GRUYTER & CO, 2017, 6(3), p. 273-279. ISSN 2191-9542. [Detail]
RUSÍN, Jiří and Kateřina CHAMRÁDOVÁ. Organic fraction of municipal solid waste and its high-solids anaerobic digestion. Waste Forum. České ekologické manažerské centrum, 2017, 4(4), p. 244-253. ISSN 1804-0195. [Detail]
KUBÁŇKOVÁ, Marie, Jaroslava HYRŠLOVÁ, Jan NEDĚLNÍK, Jiří RUSÍN and Kateřina CHAMRÁDOVÁ. Environmentální dopady zpracování komunálního odpadu. Úroda. Profi Press, 2017, nemá(12), p. 555-558. ISSN 0139-6013. [Detail]
CHAMRÁDOVÁ, Kateřina and Jiří RUSÍN. PRODUCTION OF BIOGAS THROUGH ANAEROBIC CO-FERMENTATION OF CATTLE SLURRY WITH BISCUIT WASTE. GeoScience Engineering. Vysoká škola báňská - Technická univerzita Ostrava, 2016, 62(1), p. 26-30. ISSN 1802-5420. [Detail]
RUSÍN, Jiří, Kateřina CHAMRÁDOVÁ and Kateřina KAŠÁKOVÁ. DEVELOPMENT OF THE LABORATORY ANAEROBIC BIOREACTOR FOR WET AND DRY DIGESTION PROCESSES. GeoScience Engineering. Vysoká škola báňská - Technická univerzita Ostrava, 2016, 62(4), p. 5-9. ISSN 1802-5420. [Detail]
RUSÍN, Jiří, Kateřina KAŠÁKOVÁ and Kateřina CHAMRÁDOVÁ. Anaerobic digestion of waste wafer material from the confectionery production. Energy. Elsevier, 2015, 85(1 June 2015), p. 194-199. ISSN 0360-5442. [Detail]
CHAMRÁDOVÁ, Kateřina and Jiří RUSÍN. Use of biogas biscuit meal EKPO-EB for agricultural biogas plant for substitution of energy crops utilization with organic waste. Polish journal of chemical technology. Versita, 2015, 17(3), p. 40-46. ISSN 1509-8117. [Detail]
RUSÍN, Jiří, Kateřina CHAMRÁDOVÁ and Kateřina KAŠÁKOVÁ. Fast biowaste aerobic fermentation using EWA technology in Thailand. Waste Forum. České ekologické manažerské centrum, 2015, 2(2015), p. 134-138. ISSN 1804-0195. [Detail]
CHAMRÁDOVÁ, Kateřina and Jiří RUSÍN. Možnosti využití odpadu z výroby bramborových lupínků k výrobě bioplynu. Waste Forum. České ekologické manažerské centrum, 2015, 1(2015), p. 20-25. ISSN 1804-0195. [Detail]
GRYCOVÁ, Barbora, Kateřina CHAMRÁDOVÁ, Jiří RUSÍN and Adrian PRYSZCZ. Možnosti využití odpadu z výroby instantních polévek. Waste Forum. České ekologické manažerské centrum, 2015, 2015(1), p. 26-32. ISSN 1804-0195. [Detail]
RUSÍN, Jiří, Kateřina KAŠÁKOVÁ, Kateřina CHAMRÁDOVÁ, Karel OBROUČKA and Břetislav STANĚK. High- and low-solids anaerobic digestion in laboratory model fermenter made from silage bags. Polish journal of chemical technology. Warsaw: Versita, 2013, 15(2), p. 23-28. ISSN 1509-8117. [Detail]
KAŠÁKOVÁ, Kateřina, Jiří RUSÍN and Karel OBROUČKA. Discontinuous High-Solids Anaerobic Digestion of Straw-Manure Mixtures. SGEM 2013 : 13th international multidisciplinary scientific geoconference : GeoConference on Ecology, Economics, Education and legislation : 16-22, June, 2013, Albena, Bulgaria : conference proceedings. [Book 5]. Volume I. Sofia: STEF92 Technology Ltd., 2013, 1(1), p. 283-290. ISSN 1314-2704. [Detail]
KAŠÁKOVÁ, Kateřina, Jiří RUSÍN and Karel OBROUČKA. High-solids anaerobic co-digestion of agricultural substrates in the silage bag. SGEM 2013 : 13th international multidisciplinary scientific geoconference : GeoConference on Ecology, Economics, Education and legislation : 16-22, June, 2013, Albena, Bulgaria : conference proceedings. [Book 5]. Volume I. Sofia: STEF92 Technology Ltd., 2013, 1(1), p. 539-544. ISSN 1314-2704. [Detail]
KAŠÁKOVÁ, Kateřina, Jiří RUSÍN, Kateřina CHAMRÁDOVÁ and Karel OBROUČKA. Simple Laboratory Tests of Semicontinuous High-Solids Anaerobic Digestion. Waste Forum. Praha: České ekologické manažerské centrum, 2013, 4(12.12.2013), p. 211-216. ISSN 1804-0195. [Detail]
RUSÍN, Jiří, Kateřina KAŠÁKOVÁ and Karel OBROUČKA. The yield of biogas and methane from anaerobic digestion of mixed waste of fruit and vegetables for distribution to supermarkets. 12th International Multidisciplinary Scientific GeoConference SGEM 2012 : conference proceedings. Volume I-V. Sofia: STEF92 Technology Ltd., 2012, 5(1), p. 1001-1006. ISBN 0-000-00000-0. [Detail]
RUSÍN, Jiří, Kateřina CHAMRÁDOVÁ, Karel OBROUČKA and Roman KUČA. Methane production during laboratory-scale co-fermentation of cattle slurry with 10 wt. % of various biowaste. Polish journal of chemical technology. Warsaw: Versita, 2012, 14(1), p. 14-20. ISSN 1509-8117. [Detail]
CHAMRÁDOVÁ, Kateřina, Jiří RUSÍN and Karel OBROUČKA. Modelová anaerobní kofermentace hovězí kejdy s bioodpady z výroby kávových směsí a instantních polévek. Odpadové fórum 2009 : sborník přednášek. Praha: PCHE - PetroChemEng, 2011, 2011(2), p. 67-74. ISBN 978-80-02-02108-7. [Detail]
RUSÍN, Jiří, Kateřina CHAMRÁDOVÁ and Karel OBROUČKA. Parametry digestátů z laboratorní anaerobní kofermentace hovězí kejdy s 10 % hm. různých bioodpadů z potravinářského průmyslu. Odpadové fórum 2009 : sborník přednášek. Praha: PCHE - PetroChemEng, 2011, 2011(2), p. 75-81. ISBN 978-80-02-02108-7. [Detail]
RUSÍN, Jiří, Karel OBROUČKA and Kateřina CHAMRÁDOVÁ. Měrná produkce metanu při modelové anaerobní kofermentaci směsí hovězí kejdy s 10 % hm. různých bioodpadů. Odpadové fórum 2009 : sborník přednášek. Praha: PCHE - PetroChemEng, 2010, 2010(3), p. 228-238. ISBN 978-80-02-02108-7. [Detail]
OBROUČKA, Karel, Jiří RUSÍN and Kateřina CHAMRÁDOVÁ. Modelová studie anaerobní digesce hovězí kejdy. Waste Forum. Praha: České ekologické manažerské centrum, 2010, 2010(1), p. 51-57. ISSN 1804-0195. [Detail]
OBROUČKA, Karel, Jiří RUSÍN and Kateřina CHAMRÁDOVÁ. Možnosti využití lihovarských výpalků k výrobě bioplynu. Waste Forum. Praha: České ekologické manažerské centrum, 2010, 2010(1), p. 58-67. ISSN 1804-0195. [Detail]
OBROUČKA, Karel, Jiří RUSÍN and Pavel JUREČKA. Vliv skladby vstupní směsi na efektivnost anaerobního procesu. Sborník vědeckých prací VŠB-TUO, řada vědecko-výzkumných ústavů. Ostrava: VŠB - Technická univerzita Ostrava, 2005, LI(1), p. 183-208. ISSN 1801-5514. [Detail]